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KMID : 0811720130170010099
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Korean Journal of Physiology & Pharmacology
2013 Volume.17 No. 1 p.99 ~ p.109
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Influence of Fimasartan (a Novel AT1 Receptor Blocker) on Catecholamine Release in the Adrenal Medulla of Spontaneously Hypertensive Rats
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Lim Hyo-Jeong
Lee Seog-Ki
Lim Dong-Yoon
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Abstract
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The aim of this study was to determine whether fimasartan, a newly developed AT1 receptor blocker, can affect the CA release in the isolated perfused model of the adrenal medulla of spontaneously hypertensive rats (SHRs). Fimasartan (5∼50 ?M) perfused into an adrenal vein for 90 min produced dose- and time-dependently inhibited the CA secretory responses evoked by ACh (5.32 mM), high K＋ (56 mM, a direct membrane depolarizer), DMPP (100 ?M) and McN-A-343 (100 ?M). Fimasartan failed to affect basal CA output. Furthermore, in adrenal glands loaded with fimasartan (15 ?M), the CA secretory responses evoked by Bay-K-8644 (10 ?M, an activator of L-type Ca2＋ channels), cyclopiazonic acid (10 ?M, an inhibitor of cytoplasmic Ca2＋-ATPase), and veratridine (100 ?M, an activator of Na＋ channels) as well as by angiotensin II (Ang II, 100 nM), were markedly inhibited. In simultaneous presence of fimasartan (15 ?M) and L-NAME (30 ?M, an inhibitor of NO synthase), the CA secretory responses evoked by ACh, high K＋, DMPP, Ang II, Bay-K-8644, and veratridine was not affected in comparison of data obtained from treatment with fimasartan (15 ?M) alone. Also there was no difference in NO release between before and after treatment with fimasartan (15 ?M). Collectively, these experimental results suggest that fimasartan inhibits the CA secretion evoked by Ang II, and cholinergic stimulation (both nicotininc and muscarinic receptors) as well as by membrane depolarization from the rat adrenal medulla. It seems that this inhibitory effect of fimasartan may be mediated by blocking the influx of both Na＋ and Ca2＋ through their ion channels into the rat adrenomedullary chromaffin cells as well as by inhibiting the Ca2＋ release from the cytoplasmic calcium store, which is relevant to AT1 receptor blockade without NO release.
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KEYWORD
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Adrenal Medulla, AT1 receptor blockade, Catecholamine secretion, Fimasartan
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